Fig 1: Cellular markers of T1 spontaneous lytic phenotype. (A) Volcano plots of differentially expressed genes observed from RNA sequencing of low passage, lytic BL720 (n = 3) compared to high passage, latent BL720 (n = 3) and lytic LCL720 clones (n = 3), and latent LCL720 clones (n = 3). For both of these analyses, the human genetic background is the same, so these DEGs are the result of the lytic vs latent viral phenotypes. Host DEGs are in gray, EBV DEGs are in blue. The number of genes downregulated in lytic (left side of plots) and upregulated in lytic (right side of plots) are notated. Cutoffs for DEGs are 1.5 log2FoldChange and P-adjusted <0.01. (B) GSEA analysis of BL720 RNA sequencing data revealed that GSE13411 (plasma cell vs memory B cell up) is significantly enriched in lytic BL720 compared to latent BL720. (C) Left- Genes from GSE13411 in B plotted on the BL720 volcano. Genes from the set are in blue and the genes that have >1.5 log2FoldChange and p-adjusted <0.01 are labeled. Right- the most significantly enriched genes from BL720 are plotted on the LCL720 volcano. Of these genes, SLAMF7, UCHL1, and SORT1 are significantly enriched in lytic BL720 at the >1.5 log2FoldChange and P-adjusted <0.01 cutoffs. (D) SLAMF7 (CD319) and gp350 FACS analysis in BL cells (left) and LCL720 clones (right). There is a significant reduction in SLAMF7 surface expression in high passage, latent BL720 compared to low passage, lytic BL720. Likewise, there is a significant decrease in expression in the latent LCL720 clones compared to the lytic LCL720 clones (***P < 0.0006). Representative FACS plots from these analyses are displayed at the bottom.